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Cell Sorters

BD Influx (BPS - Rm 5115)                                                            Assisted Sorting $45/hr

The BD Influx Sorter can detect up to 14 colors with a maximum of 16 parameters. Forward scatter is collected from a high sensitivity detector unit with resolution slightly below 0.2 μm.

  • Installed in a Baker BioProtect IV Biosafety Cabinet to allow for sorting of BSL-2 samples.
  • Sorting up to 50x106 cells/hour (cell-type dependent).
  • 70, 100, 130, and 200 µm nozzles available.
  • Up to 6 populations sorted simultaneously into Eppendorf tubes, 5 mL, or 15 mL conical tubes.
  • Populations can also be sorted directly into multi-well plates, up to 384-well.
  • 5 laser system (up to 14 fluorescent parameters):
    • UV 355nm – Hoechst, Indo-1, AF350, BUV dyes
    • Violet 405nm – AF405, BV dyes, Pac Blue, Pac Orange
    • Blue 488nm – GFP, FITC, YFP, PerCp-Cy5.5 (FSC PMT)
    • Y-G 552nm – PE, mCherry, tdTomato, fruit dyes, PE-Cy7
    • Red 633nm – APC, AF700, APC-Cy7

Scientist working at the BD Influx Sorter

BD Influx Instrument Configuration

 


BD FACSAria IIu (IQ - Rm 2522)                                           Assisted Sorting $129.68/hr

The BD FACSAria IIu is the original model FACSAria-I cell sorter that has been upgraded with a FACSAria II flow cell and fluidics.

  • Installed in a Baker BioProtect IV Biosafety Cabinet to allow for sorting of BSL-2 samples.
  • Sorting up to 50x106 cells/hour (cell-type dependent).
  • 70, 85, & 100 µm nozzles available.
  • Up to 4 populations sorted simultaneously into Eppendorf tubes, 5 mL, or 15 mL conical tubes.
  • Populations can also be sorted directly into multi-well plates, up to 96-well.
  • 3 laser system (up to 13 fluorescent parameters):
    • Violet 405nm - 100 mW laser (4 detectors)
    • Blue 488nm - 50 mW laser (6 detectors + FSC/SSC)
    • Red 633nm - 100 mW laser (3 detectors)

Picture of BD FACS ARIA llu machine

BD FACSAria IIu Configuration