Cytek Aurora (IQ - Rm 2521) Unassisted use $31.69/hr
The Cytek Aurora is a spectral flow cytometer equipped with 5 lasers (UV 355nm, violet 405nm, blue 488nm, yellow-green 561nm, and red 640nm) and has the capability of detecting up to 64 fluorescence channels with additional light scatter detectors off of the violet (SSC) and blue lasers (FSC and SSC). The optics and state-of-the-art low-noise electronics provide excellent sensitivity and resolution. The instrument is also equipped with an auto-sampler for sample acquisition in 96-well plates.
Cytek Aurora website: https://cytekbio.com/pages/aurora
BD Accuri C6 (IQ - Rm 2521) Unassisted use $27.79/hr
The BD Accuri C6 is a compact flow cytometer that uses a low-pressure pumping system to drive the fluidics allowing for the derivation of sample volume and calculation of absolute counts or sample concentration per microliter. The instrument is capable of running up to 10,000 events per second at sample concentrations of over 5 x 106 cells/mL. It is equipped with 2 lasers: a blue 488nm (3 detectors + FSC/SSC; FL1, FL2, and FL3) and a red 640nm (1 detector; FL-4). The co-linear optical configuration allows multi-parameter detection of up to 4 fluorescent parameters and 2 light scatter parameters. Optical filters allow for detection of commonly used fluorescent markers, including, but not limited to:
- FL1 - FITC, AF488, GFP, CFSE, YFP [530 ± 15 nm]
- FL2 - PE, PI [585 ± 20 nm]
- FL3 - PerCP, PerCP-Cy5.5, PE-Cy7, RFP, mCherry [>670 nm]
- FL-4 - APC, AF647, APC-Cy7, APC-H7 [675 ± 12.5 nm]
BD LSR II (BPS - Rm 5115) Unassisted use $30/hr
The BD LSR II can detect up to 11 parameters, including 9 fluorescent parameters, and is used for analysis only. The laser excitation lines and common fluorochromes excited are:
- Violet 405nm - Pacific Blue, BV421, AmCyan, BV510
- Blue 488nm - FITC, PE, PerCp-Cy5.5, PE-Cy7. Other fluorochrome combinations are available for the PerCP-Cy5 detector. GFP, YFP or RFP reporter proteins can also be detected.
- Red 640nm - APC, Alexa 700, APC-Cy7