Instrumentation

  • FL1- FITC, AF488, GFP, CFSE, YFP FL2- PE, PI FL3- PerCP, PerCP-Cy5.5, PE-Cy7, RFP, mCherry FL4- APC, AF647, APC-Cy7, APC-H7

Cytek Aurora

The Cytek Aurora is a spectral flow cytometer equipped with 4 lasers (violet 405nm, blue 488nm, yellow-green 561nm, and red 640nm) and has the capability of detecting up to 48 fluorescence channels with additional light scatter detectors off of the violet laser (SSC) and blue laser (FSC). The optics and state-of-the-art low-noise electronics provide excellent sensitivity and resolution. The Cytek Aurora is located in the MSU South Campus Flow Cytometry Core (Room 2521, IQ Building). Future upgrades will include a 96-well auto-sampler and a UV laser.

Cytek Aurora

Cytek Aurora website: https://cytekbio.com/pages/aurora

BD FACS Aria IIu

The BD FACS Aria IIu is the original model FACS Aria-I cell sorter that has been upgraded with a FACS Aria II flow cell.  It is equipped with 3 lasers: a blue 488nm laser (6 detectors + FSC/SSC), a red 633nm laser (3 detectors), and a violet 405nm laser (4 detectors).  The optical configuration allows multi-parameter detection of up to 13 fluorescent parameters and 2 light scatter parameters. The FACS Aria-IIu is capable of sorting through over 50 million cells per hour (dependent on cell type), with up to four populations simultaneously. The populations of interest can be sorted into 0.5 mL/1 mL Eppendorf tubes, 5 mL tubes, or 15 mL conical tubes.  Populations can also be sorted directly into multi-well plates, including: 12-well, 24-well, 48-well, 96-well.

Picture of BD FACS ARIA llu machineBD FACS Aria llu machine specs

BD Accuri C6

The BD Accuri C6 is a compact flow cytometer that uses a low-pressure pumping system to drive the fluidics allowing for the derivation of sample volume and calculation of absolute counts or sample concentration per microliter. The instrument is capable of running up to 10,000 events per second at sample concentrations of over 5 x 106 cells/mL. It is equipped with 2 lasers: a blue 488nm (3 detectors + FSC/SSC; FL1 [530±15 nm], FL2 [585±20 nm], FL3 [>670 nm]) and a red 640nm (1 detector; FL-4 [675±12.5 nm]). The optical configuration allows multi-parameter detection of up to 4 fluorescent parameters and 2 light scatter parameters. Optical filters allow for detection of commonly used fluorescent markers, including, but not limited to:

  • FL1- FITC, AF488, GFP, CFSE, YFP

  • FL2- PE, PI

  • FL3- PerCP, PerCP-Cy5.5, PE-Cy7, RFP, mCherry

  • FL4- APC, AF647, APC-Cy7, APC-H7

BD Accuria C6 machine

Luminex 200

The Luminex 200 is a diagnostic instrument that enables bead-based multiplex analysis of up to 100 analytes simultaneously in a single low-volume (≥ 2 µL) sample. This system utilizes fluorescently labeled microbeads that are conjugated with a specific reagent (eg, antibody, antigen, enzyme substrate, etc.) enable capture and detection of analytes of interest. The Luminex 200 is equipped with 2 lasers: a 635nm classification laser for excitation of fluorescently-labeled microbeads and a 532nm reporter laser for excitation of the analyte signal. The Luminex 200 has a dynamic range typically ≥ 3.5 logs.

Commercially available and custom multiplex kits can be analyzed on the Luminex 200 to quantify protein expression (ie, cytokines, growth factors, phosphoproteins, etc.) in a variety of species (ie, human, mouse, rat, dog, etc.) and samples types (ie, cell culture media, plasma, serum, BALF, etc.). In addition to immunoassays, the platform can be used for enzyme assays, genotyping, gene expression analysis, or assessment of protein-protein interactions.

A handheld magnetic plate washer, microtiter plate shaker, bath sonicator, and vortexer are available in the core to enable multiplex assay performance.

Luminex 200 machine