The Cytek Aurora is a spectral flow cytometer equipped with 5 lasers (UV 355nm, violet 405nm, blue 488nm, yellow-green 561nm, and red 640nm) and has the capability of detecting up to 64 fluorescence channels with additional light scatter detectors off of the violet laser (SSC) and blue laser (FSC and SSC). The optics and state-of-the-art low-noise electronics provide excellent sensitivity and resolution. The instrument is also equipped with an auto-sampler for sample acquisition in 96-well plates. The Cytek Aurora is located in the MSU South Campus Flow Cytometry Core (Room 2521, IQ Building).
Cytek Aurora website: https://cytekbio.com/pages/aurora
BD Accuri C6
The BD Accuri C6 is a compact flow cytometer that uses a low-pressure pumping system to drive the fluidics allowing for the derivation of sample volume and calculation of absolute counts or sample concentration per microliter. The instrument is capable of running up to 10,000 events per second at sample concentrations of over 5 x 106 cells/mL. It is equipped with 2 lasers: a blue 488nm (3 detectors + FSC/SSC; FL1 [530±15 nm], FL2 [585±20 nm], FL3 [>670 nm]) and a red 640nm (1 detector; FL-4 [675±12.5 nm]). The optical configuration allows multi-parameter detection of up to 4 fluorescent parameters and 2 light scatter parameters. Optical filters allow for detection of commonly used fluorescent markers, including, but not limited to:
FL1- FITC, AF488, GFP, CFSE, YFP
FL2- PE, PI
FL3- PerCP, PerCP-Cy5.5, PE-Cy7, RFP, mCherry
FL4- APC, AF647, APC-Cy7, APC-H7
BD FACS Aria IIu
The BD FACS Aria IIu is the original model FACS Aria-I cell sorter that has been upgraded with a FACS Aria II flow cell. It is equipped with 3 lasers: a blue 488nm laser (6 detectors + FSC/SSC), a red 633nm laser (3 detectors), and a violet 405nm laser (4 detectors). The optical configuration allows multi-parameter detection of up to 13 fluorescent parameters and 2 light scatter parameters. The FACS Aria-IIu is capable of sorting through over 50 million cells per hour (dependent on cell type), with up to four populations simultaneously. The populations of interest can be sorted into 0.5 mL/1 mL Eppendorf tubes, 5 mL tubes, or 15 mL conical tubes. Populations can also be sorted directly into multi-well plates, including: 12-well, 24-well, 48-well, 96-well.
The Luminex 200 is a diagnostic instrument that enables bead-based multiplex analysis of up to 100 analytes simultaneously in a single low-volume (≥ 2 µL) sample. This system utilizes fluorescently labeled microbeads that are conjugated with a specific reagent (eg, antibody, antigen, enzyme substrate, etc.) enable capture and detection of analytes of interest. The Luminex 200 is equipped with 2 lasers: a 635nm classification laser for excitation of fluorescently-labeled microbeads and a 532nm reporter laser for excitation of the analyte signal. The Luminex 200 has a dynamic range typically ≥ 3.5 logs.
Commercially available and custom multiplex kits can be analyzed on the Luminex 200 to quantify protein expression (ie, cytokines, growth factors, phosphoproteins, etc.) in a variety of species (ie, human, mouse, rat, dog, etc.) and samples types (ie, cell culture media, plasma, serum, BALF, etc.). In addition to immunoassays, the platform can be used for enzyme assays, genotyping, gene expression analysis, or assessment of protein-protein interactions.
A handheld magnetic plate washer, microtiter plate shaker, bath sonicator, and vortexer are available in the core to enable multiplex assay performance.