Toggle Accessibility Tools

Instrumentation

Instrument training, use of benchtop analyzers, and sorting services can be scheduled through the iLab Solutions platform.


Cytek Aurora (IQ - Rm 2521)                                                     Unassisted use $31.69/hr 

The Cytek Aurora is a spectral flow cytometer equipped with 5 lasers (UV 355nm, violet 405nm, blue 488nm, yellow-green 561nm, and red 640nm) and has the capability of detecting up to 64 fluorescence channels with additional light scatter detectors off of the violet (SSC) and blue lasers (FSC and SSC). The optics and state-of-the-art low-noise electronics provide excellent sensitivity and resolution. The instrument is also equipped with an auto-sampler for sample acquisition in 96-well plates

Cytek Aurora

Cytek Aurora website: https://cytekbio.com/pages/aurora


BD Accuri C6 (IQ - Rm 2521)                                                     Unassisted use $27.79/hr 

The BD Accuri C6 is a compact flow cytometer that uses a low-pressure pumping system to drive the fluidics allowing for the derivation of sample volume and calculation of absolute counts or sample concentration per microliter. The instrument is capable of running up to 10,000 events per second at sample concentrations of over 5 x 106 cells/mL. It is equipped with 2 lasers: a blue 488nm (3 detectors + FSC/SSC; FL1, FL2, and FL3) and a red 640nm (1 detector; FL-4). The co-linear optical configuration allows multi-parameter detection of up to 4 fluorescent parameters and 2 light scatter parameters. Optical filters allow for detection of commonly used fluorescent markers, including, but not limited to:

  • FL1 - FITC, AF488, GFP, CFSE, YFP [530 ± 15 nm]
  • FL2 - PE, PI [585 ± 20 nm]
  • FL3 - PerCP, PerCP-Cy5.5, PE-Cy7, RFP, mCherry  [>670 nm]
  • FL-4 - APC, AF647, APC-Cy7, APC-H7  [675 ± 12.5 nm]

BD Accuria C6 machine

 


BD LSR II (BPS - Rm 5115)                                                              Unassisted use $30/hr 

The BD LSR II can detect up to 11 parameters, including 9 fluorescent parameters, and is used for analysis only. The laser excitation lines and common fluorochromes excited are:

  • Violet 405nm - Pacific Blue, BV421, AmCyan, BV510
  • Blue 488nm - FITC, PE, PerCp-Cy5.5, PE-Cy7. Other fluorochrome combinations are available for the PerCP-Cy5 detector. GFP, YFP or RFP reporter proteins can also be detected.
  • Red 640nm - APC, Alexa 700, APC-Cy7

BD LSR 2

BD LSR II Instrument Configuration

 
 

​​​​



BD Influx (BPS - Rm 5115)                                                            Assisted Sorting $45/hr

The BD Influx Sorter can detect up to 14 colors with a maximum of 16 parameters. Forward scatter is collected from a high sensitivity detector unit with resolution slightly below 0.2 μm.

  • Installed in a Baker BioProtect IV Biosafety Cabinet to allow for sorting of BSL-2 samples.
  • Sorting up to 50x106 cells/hour (cell-type dependent).
  • 70, 100, 130, and 200 µm nozzles available.
  • Up to 6 populations sorted simultaneously into Eppendorf tubes, 5 mL, or 15 mL conical tubes.
  • Populations can also be sorted directly into multi-well plates, up to 384-well.
  • 5 laser system (up to 14 fluorescent parameters):
    • UV 355nm – Hoechst, Indo-1, AF350, BUV dyes
    • Violet 405nm – AF405, BV dyes, Pac Blue, Pac Orange
    • Blue 488nm – GFP, FITC, YFP, PerCp-Cy5.5 (FSC PMT)
    • Y-G 552nm – PE, mCherry, tdTomato, fruit dyes, PE-Cy7
    • Red 633nm – APC, AF700, APC-Cy7

Scientist working at the BD Influx Sorter

BD Influx Instrument Configuration

 

BD FACSAria IIu (IQ - Rm 2522)                                           Assisted Sorting $129.68/hr

The BD FACSAria IIu is the original model FACSAria-I cell sorter that has been upgraded with a FACSAria II flow cell and fluidics.

  • Installed in a Baker BioProtect IV Biosafety Cabinet to allow for sorting of BSL-2 samples.
  • Sorting up to 50x106 cells/hour (cell-type dependent).
  • 70, 85, & 100 µm nozzles available.
  • Up to 4 populations sorted simultaneously into Eppendorf tubes, 5 mL, or 15 mL conical tubes.
  • Populations can also be sorted directly into multi-well plates, up to 96-well.
  • 3 laser system (up to 13 fluorescent parameters):
    • Violet 405nm - 100 mW laser (4 detectors)
    • Blue 488nm - 50 mW laser (6 detectors + FSC/SSC)
    • Red 633nm - 100 mW laser (3 detectors)

Picture of BD FACS ARIA llu machine

BD FACSAria IIu Configuration

 


Luminex 200 (IQ - Rm 2522)                                                 Assisted setup $104.69/use

The Luminex 200 is a diagnostic instrument that enables bead-based multiplex analysis of up to 100 analytes simultaneously in a single low-volume (≥ 2 µL) sample. This system utilizes fluorescently labeled microbeads that are conjugated with a specific reagent (eg, antibody, antigen, enzyme substrate, etc.) enable capture and detection of analytes of interest. The Luminex 200 is equipped with 2 lasers: a 635nm classification laser for excitation of fluorescently-labeled microbeads and a 532nm reporter laser for excitation of the analyte signal. The Luminex 200 has a dynamic range typically ≥ 3.5 logs.

Commercially available and custom multiplex kits can be analyzed on the Luminex 200 to quantify protein expression (ie, cytokines, growth factors, phosphoproteins, etc.) in a variety of species (ie, human, mouse, rat, dog, etc.) and samples types (ie, cell culture media, plasma, serum, BALF, etc.). In addition to immunoassays, the platform can be used for enzyme assays, genotyping, gene expression analysis, or assessment of protein-protein interactions.

A handheld magnetic plate washer, microtiter plate shaker, bath sonicator, and vortexer are available in the core to enable multiplex assay performance.

Luminex 200 machine